1122. Incidence and Spa-types of Penicillin-susceptible Bacteremic Staphylococcus aureus Isolates in Southern Sweden 2014
Session: Poster Abstract Session: MRSA/VRE Epidemiology
Friday, October 9, 2015
Room: Poster Hall
  • Poster IDWeek 1122.pdf (271.5 kB)
  • Background: Bensylpenicillin has a distinct treatment advantage over cloxacillin against Staphylococcus aureus (SA) that do not produce penicillinase, providing longer T>MIC using normal antibiotic dosing regimes. Such isolates have, however, been uncommon and methods of identifying this subgroup, such as chromogenic cephalosporins, have been deceptive since the penicillinase is not constitutionally expressed but inducible. Due to this, susceptibility to penicillin is not generally tested in Sweden. In the present study, we aimed to determine the local rates and molecular types of penicillin-susceptible S. aureus (PSSA) as well as to investigate the usefulness of current methods to screen for PSSA isolates.

    Methods:   All S. aureus isolated from blood cultures in southwestern Skåne county (population approx. 500,000), Sweden in 2014 were subjected to susceptibility testing using E-tests for bensylpenicillin and disk diffusion with PcG (1U), and finally the chromogenic nitrocephin test for betalactamase. All isolates with a negative nitrocephin test and that were determined as susceptible according to EUCAST were subjected to a PCR detecting the S. aureus penicillinase as well as to specific S. aureus protein A (spa) typing.

    Results: During 2014, 275 S. aureus isolates were isolated from blood in our catchment area. A total of 85 (31%) of the isolates were likely penicillin-susceptible according to aforementioned criteria. In these isolates, a penicillinase was detected in only a few cases, leaving the true incidence of penicillin-susceptible isolates just below 30%. Spa-typing suggested that while penicillin-susceptible isolates are not clonal, they are limited to a restricted number of spa types.

    Conclusion:   Penicillin-susceptible S. aureus are more common in Sweden than previously reported, and testing for penicillin susceptibility should be performed more often in clinical practice. The current EUCAST methodology for detection of susceptibility is a good, but not perfect, predictor of true penicillin-susceptibility.

    Fredrik Resman, MD PhD1,2, Johan Tham, MD, PhD1, Jonas Ahl, MD, PhD1, Fredrik Månsson, MD, PhD1 and Kristian Riesbeck, professor2, (1)Infectious Diseases Research Unit, Department of Clinical Sciences, Lund University, Malmö, Sweden, (2)Clinical Microbiology, Department of Translational Medicine, Lund University, Malmö, Sweden


    F. Resman, None

    J. Tham, None

    J. Ahl, None

    F. Månsson, None

    K. Riesbeck, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 7th with the exception of research findings presented at the IDWeek press conferences.