Methods: We obtained 34 bone samples from patients admitted to our hospital with a moderate to severe diabetic foot infection. We analysed the distribution of the 16S rRNA gene sequences in the bone samples, using an Illumina MiSeq Personal Sequencer. We compared the genera that were detected with the cultured pathogens in the bone samples with conventional techniques.
Results: Staphylococcus spp. was identified in all of the sequenced bone samples that were negative with conventional techniques. Mixed genera were present in 83.3% (5 of 6) of the negative samples. In the 23 samples that had positive results with both techniques, Staphylococcus, Corynebacterium, Streptococcus and Propionibacterium spp. were detected in 20, 18, 13, and 11 samples respectively. More anaerobes were detected with 16S rRNA sequencing compared to conventional techniques (86.9% vs 23.1%) and more polymicrobial infections were present (91.3% vs 64.0%).
Conclusion: Anaerobic and fastidious organisms may play a bigger role in osteomyelitis than previously reported. Further studies with bigger populations are needed to understand the clinical importance of the microbial diversity of diabetic foot osteomyelitis.
S. Van Asten,
E. Peters, None
K. Bhavan, None
P. Kim, None
L. Lavery, None
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