1789. Emergence of New Delhi Metallo-β Lactamase (NDM 1) Encoding Gene among Gram Negative Bacteria Isolated from Hospital Effluents
Session: Poster Abstract Session: Resistant Gram-Negative Infections: CRE Epidemiology
Saturday, October 10, 2015
Room: Poster Hall
  • Veena Shetty NDM.pdf (665.0 kB)
  • Background: The emergence and spread of New Delhi metallo-β – Lactamase variant 1 (NDM-1), a broad spectrum β lactamase (carbapenemase) which inactivate all β – lactams except aztreonam is a major concern worldwide. The spread of multi-drug resistant NDM-1 through hospital wastes and their release into surrounding environment may favor the horizontal transfer of genes to other microbial community rapidly. We studied the presence of NDM-1 gene among Gram negative bacteria (GNB) isolated from untreated hospital effluents. 

    Methods: During the period of one year from July 2013-July 2014, we collected GNB isolates from untreated effluents samples of two tertiary care hospitals in Mangalore, South India. All isolates were subjected for antibiotic susceptibility test by Kirby Bauer method. Antibiogram analysis was done. The isolates showing resistant to carbapenem group (imipenem or meropenem) were selected and DNA was extracted. After DNA extraction, isolates were tested for the presence of bla NDM-1 gene using PCR and were confirmed by sequencing the gene. MIC was determined by E strip method for tetracycline, cotrimaxazole, ciprofloxacin, meropenem, nitrofurantoin, chloramphenicol, cefotaxime. 

    Results: A total of 45 effluent samples were collected and 414 GNB were isolated. Of the 414 isolates, 341 were found to be multidrug resistant. Out of 220 carbapenem-resistant isolates, 22 isolates were positive for blaNDM-1 (E. coli 7, Klebsiella spp 6, Pseudomonas spp 6, Enterobacter 1, Proteus spp 1 and Shigella spp 1). Isolates sent for sequencing matched with the sequences of gene bank in a range of 96 - 99%.

    Conclusion: To minimize the risk of rapid dissemination of this multi-drug resistant  emerging pathogen to the community and environment, indiscriminate usage of antibiotics should be controlled in hospitals along with adapting appropriate effluent treatment strategies.

    Veena Shetty, Ph.D, Microbiology, K.S.Hegde Med. Academy, Nitte Univ., Mangalore, India, Divyashree M, M.Sc, Microbiology, Nitte Univ., Mangalore, India, Deekshit Vijay Kumar, PhD, Biomedical Science, Nitte Univ., Mangalore, India, Avinash Shetty, MD, FIDSA, Wake Forest University School of Medicine, Winston-Salem, NC and Indrani Karunasagar, PhD, Biomedical Science, Nitte University Center for Science Education and Research (NUCSER), Mangalore, India


    V. Shetty, None

    D. M, None

    D. Vijay Kumar, None

    A. Shetty, None

    I. Karunasagar, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 7th with the exception of research findings presented at the IDWeek press conferences.