1603. Comparison of Methods for Surveillance Culture of Carbapenem-Resistant Acinetobacter baumannii
Session: Poster Abstract Session: ESBL and CRE Diagnostics
Saturday, October 10, 2015
Room: Poster Hall
Background: Early identification of patients colonized with carbapenem-resistant Acinetobacter baumannii (CRAB) is important in reducing the nosocomial spread of CRAB. This study was performed to determine the most effective method for surveillance cultures of CRAB.

Methods: Nasal and rectal swabs were obtained concurrently from 37 intensive care patients colonized with CRAB. All 74 samples were inoculated in chromogenic medium (CHROMagarTM Acinetobacter with selective supplement CR102), MacConkey agar supplemented with 5 μg/mL imipenem (MAC-IPM), and Tryptic Soy broth supplemented with 5 μg/mL imipenem (TSB-IPM). CRAB detection rates from each sample were determined and compared.

Results: Using CHROMagar, MAC-IPM, and TSB-IPM, CRAB detection rate from nasal swabs was 67.6% (25/37), 73.0% (27/37), and 78.4% (29/37), respectively. The respective detection rates from rectal swabs were 54.1% (20/37), 48.6% (18/37), and 54.1% (20/37). Respective detection rates from either nasal or rectal swabs obtained from 37 patients were 89.2% (33/37), 78.4% (29/37), and 86.5% (32/37). While there was no significant difference in the CRAB detection rates between the three different selective media, CHROMagar allowed the presumptive identification of CRAB in 40 (88.9%) of 45 isolates within 24 hours.

Conclusion: CHROMagarTM Acinetobacter using specimens obtained concurrently from nasal and rectal swabs is the most effective method for CRAB surveillance culture.

Han-Sung Kim, MD1, Seung Soon Lee, MD2, Jeong-a Lee, MD2 and Jae-Seok Kim, MD1, (1)Laboratory Medicine, Hallym University Medical Center, Anyang, South Korea, (2)Infectious Diseases, Hallym University Medical Center, Anyang, South Korea

Disclosures:

H. S. Kim, None

S. S. Lee, None

J. A. Lee, None

J. S. Kim, None

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