Methods: We prospectively evaluated two real-time polymerase chain reaction (PCR) assays and seroconversion of IgG Antibody for Rickettsia spp. and Bartonella spp. infection among the patients with AUF in Southern Thailand from January 2013 to December 2014.
Results: Of 150 patients with AUF, 40 (26.7%) patients had seroconversion of Rickettsia spp. infection while there was no seroconversion for Bartonella spp. infection. By seroconversion , 7.3% (11 of 150) and 18 % (27 of 150) of patients were confirmed to have scrub or murine typhus, respectively. There was 2 patients had unidentified specific antibody for Rickettsia spp. infection. The PCR assays for the detection of O. tsutsugamushi and Rickettsia spp. had high sensitivity and specificity (97.5 % [95% confidence interval (CI): 95.2–100] and 100% [95% CI: 97.8–100], respectively). For those 2 patients, a PCR to detect the gltA of the Rickettsia feliswere positive in their acute serum. The PCR products were also confirmed with sequencing analysis.
Conclusion: Orientia tsutsugamushi and Rickettsia typhi were the major causes of Rickettsia spp. infection in southern Thailand. This is the first report of R. felis infection in human from southern Thailand. Surveillance for Rickettsia spp. is needed due to the emergence of novel species.
B. Thaisomboonsuk, None