878. Human Genetic Variation in VAC14 Alters Susceptibility to S. typhi Infection
Session: Poster Abstract Session: Bacterial Infections: Pathogenesis and Immunity
Friday, October 9, 2015
Room: Poster Hall
Posters
  • Alvarez_MGMretreatposterVfinal.pdf (1.6 MB)
  • Background: Human genetic variation influences many aspects of our lives including susceptibility to infectious diseases. Through a novel cell-based system screen of human genetic variation (Hi-HOST: High-throughput human in vitro susceptibility testing), our lab has discovered a human genetic variant in the scaffolding protein VAC14, which affects Salmonella infection in lymphoblastoid cell lines.

    Methods: Through genetic manipulation and the use of chemical inhibitors we altered the levels of VAC14 in the cell. Additional experiments were done using confocal microscopy and flow cytometric analysis. 

    Results: Through RNAi, chemical inhibitors and CRISPRs we confirm the genetic analysis that VAC14 affects Salmonella invasion.

    Conclusion: Human genetic variation may play a larger role in disease susceptibility than previously expected. We show here that a SNP in VAC14 can have very different effects on Salmonella invasion and thus susceptibility to Typhoid Fever.

    Monica Thomas, PhD Candidate, Molecular Genetics and Microbiology, Duke University, DURHAM, NC

    Disclosures:

    M. Thomas, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 7th with the exception of research findings presented at the IDWeek press conferences.