878. Human Genetic Variation in VAC14 Alters Susceptibility to S. typhi Infection
Session: Poster Abstract Session: Bacterial Infections: Pathogenesis and Immunity
Friday, October 9, 2015
Room: Poster Hall
  • Alvarez_MGMretreatposterVfinal.pdf (1.6 MB)
  • Background: Human genetic variation influences many aspects of our lives including susceptibility to infectious diseases. Through a novel cell-based system screen of human genetic variation (Hi-HOST: High-throughput human in vitro susceptibility testing), our lab has discovered a human genetic variant in the scaffolding protein VAC14, which affects Salmonella infection in lymphoblastoid cell lines.

    Methods: Through genetic manipulation and the use of chemical inhibitors we altered the levels of VAC14 in the cell. Additional experiments were done using confocal microscopy and flow cytometric analysis. 

    Results: Through RNAi, chemical inhibitors and CRISPRs we confirm the genetic analysis that VAC14 affects Salmonella invasion.

    Conclusion: Human genetic variation may play a larger role in disease susceptibility than previously expected. We show here that a SNP in VAC14 can have very different effects on Salmonella invasion and thus susceptibility to Typhoid Fever.

    Monica Thomas, PhD Candidate, Molecular Genetics and Microbiology, Duke University, DURHAM, NC


    M. Thomas, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 7th with the exception of research findings presented at the IDWeek press conferences.