878. Human Genetic Variation in VAC14 Alters Susceptibility to S. typhi Infection
Session: Poster Abstract Session: Bacterial Infections: Pathogenesis and Immunity
Friday, October 9, 2015
Room: Poster Hall
Background: Human genetic variation influences many aspects of our lives including susceptibility to infectious diseases. Through a novel cell-based system screen of human genetic variation (Hi-HOST: High-throughput human in vitro susceptibility testing), our lab has discovered a human genetic variant in the scaffolding protein VAC14, which affects Salmonella infection in lymphoblastoid cell lines.
Methods: Through genetic manipulation and the use of chemical inhibitors we altered the levels of VAC14 in the cell. Additional experiments were done using confocal microscopy and flow cytometric analysis.
Results: Through RNAi, chemical inhibitors and CRISPRs we confirm the genetic analysis that VAC14 affects Salmonella invasion.
Conclusion: Human genetic variation may play a larger role in disease susceptibility than previously expected. We show here that a SNP in VAC14 can have very different effects on Salmonella invasion and thus susceptibility to Typhoid Fever.
Monica Thomas, PhD Candidate, Molecular Genetics and Microbiology, Duke University, DURHAM, NC