Methods: We performed a cross-sectional survey of C. difficile positive samples from Saint Joseph Mercy Hospital from February 3rd, 2015 to May 12th, 2015. We measured the amount of toxin in stool using an Enzyme Immunoassay (EIA) (Techlab: C. difficileToxA/B II). We also quantified the number of spores by culturing serial dilutions of each specimen following a heat shock.
Results: To date, we have screened 115 samples--82 inpatients, 30 outpatients and 3 from the Emergency Department. Our study population was composed of 69 females (60%) and 63 patients (55%) were above 65 years of age. In contrast to in vitro results, in vivo spore and toxin levels were positively correlated (r=0.627, p = <0.001). There is also a trend suggesting correlation of age with spore levels (r= 0.213, p= 0.022) and toxin levels(r= 0.152, p= 0.106).
Conclusion: Among stool samples positive for C.difficile, spore and toxin levels were positively correlated. This suggests that resolving CDI symptoms may correspond to decreasing infectivity, and that, in vivo, there is no trade off for C.difficile between sporulation and toxin production.
M. Eisenberg, None
A. Rickard, None
S. Walk, None
B. Foxman, None
See more of: Poster Abstract Session