945. Characteristics of fecal microbiota in Korean patients with Clostridium difficile associated diarrhea
Session: Poster Abstract Session: Clostridium difficile Infections: Epidemiology and Diagnostics
Friday, October 9, 2015
Room: Poster Hall
Background: The intestinal microbiota plays an important role in the pathogenesis of Clostridium difficile (C.difficile) associated diarrhea and regional and racial characteristics influence the composition and diversity of the microbiota. We investigated intestinal microbiome characteristics in patients with C.difficile enterocolitis comparing with other colitis, patients with VRE colonization and healthy controls.

Methods: We collected stool samples from 14 patients with C.difficile colitis (CD+), 8 patients with colitis but not from C.difficile (CD-), 11 patients with VRE colonization (VRE) and 10 healthy controls (Healthy). The microbial communities were evaluated by 454-pyrosequencing of bacterial 16s rRNA.   

Results: : Mean age of participants was 54.6±18.3, and 47% of participants was male. Number of OTUs and diversity (by Chao 1 estimator, p=0.001) of CD+ group were significantly decreased compared with healthy control. VRE group showed lower number of OTUs and diversity than CD+ group. CD+ and CD- group had no significant difference in number of OTUs and diversity. In CD+ stool, the proportion of Bifidobacteriales (contains genus Bifidobacterium, p=0.016), and Clostridiales (contains genus Lachnospiraceae, Ruminococcacea and Clostridium VIII) families were significantly reduced by comparison with healthy control. Compared with CD- group, CD+ samples had more Enterobacteriales order and Hungatella, Clostridium IV among Clostridiales family. In comparison with VRE group, CD+ group showed less Lactobacillales and more Coriobacterialesorder.

Conclusion: There were distinguishing features of fecal microbiota in Korean patients with C. difficile associated diarrhea. The metagenomic analyses would be helpful for developing strategies to predict and diagnose C. difficile associated diarrhea.

Hea Won Ann, MD1, Jin Young Ahn, MD1, Young Soun Lim, M.S2, Mi-Young Ahn, MD1, Yong Duk Jeon, MD1, In Young Jung, MD1, Nam Su Ku, MD1, Dongeun Yong, MD, PhD3, Kyungwon Lee, MD, PhD3, June Myung Kim, MD, PhD1 and Jun Yong Choi, MD, PhD1, (1)Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea, (2)AIDS Research Institute, Yonsei University College of Medicine, Seoul, South Korea, (3)Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea

Disclosures:

H. W. Ann, None

J. Y. Ahn, None

Y. S. Lim, None

M. Y. Ahn, None

Y. D. Jeon, None

I. Y. Jung, None

N. S. Ku, None

D. Yong, None

K. Lee, None

J. M. Kim, None

J. Y. Choi, None

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