Methods: Human immunodeficiency virus negative subjects with smear-positive drug-susceptible PTB were enrolled prior to initiation of ATT at the Grady Memorial Hospital, Atlanta, GA and followed longitudinally during ATT. ATT was provided according to national guidelines. Blood samples were obtained at baseline and during ATT. Peripheral blood mononuclear cells (PBMC) and plasma were isolated for each patient at each time point. Plasma IP-10 levels were measured by ELISA.
Results: All patients achieved sputum culture-conversion and were considered cured. In plasma samples for four patients tested to date, IP-10 levels decreased with ATT. The decrease in IP-10 mirrored decreases in frequencies of CD38+, HLA-DR+, and Ki-67+ M. tuberculosis-specific CD4+IFN-γ+ T cells. These markers of T cell activation and proliferation were previously shown by our group to be biomarkers of treatment response.
Conclusion: In our limited sample set, the trend of decreasing plasma IP-10 during ATT appears to mirror the decrease in M. tuberculosis specific T cell markers of treatment response.
M. C. Schechter,
T. Adekambi, None
S. Cagle, None
S. M. Ray, None
J. Rengarajan, None