1798. An Outbreak of NDM-1 Klebsiella pneumoniae in a Tertiary Care Hospital in British Columbia The Role of Molecular Analysis in Determining Transmission
Session: Poster Abstract Session: Resistant Gram-Negative Infections: CRE Epidemiology
Saturday, October 10, 2015
Room: Poster Hall
  • CRE outbreak Sep29 draft.pdf (664.6 kB)
  • Background:

    Carbapenemase-producing organisms (CPO) are considered a growing healthcare problem. We are reporting an outbreak of a New Delhi metallo-beta-lactamase (NDM-1) producing Klebsiella pneumoniae that occurred at a major tertiary care facility in British Columbia from November 2013 to March 2014.


    Gram negative bacilli from clinical and surveillance specimens were tested for carbapenem susceptibility by VITEK 2. Carbapenem non-susceptible organisms were further tested by E-test and ROSCO Neo-SensitabsTM. PCR for carbapenemase genes was done on all carbapenem non-susceptible isolates. Pulsed field gel electrophoresis (PFGE) was performed on the isolates. Plasmid restriction fragment length polymorphism (RFLP) was done for carbapenemase producing plasmids using BglII.


    A total of eleven patients were considered part of the K. pneumoniae NDM-1 outbreak on a medical unit. PFGE showed that the patients’ isolates had 2 main clonal patterns. Plasmid analysis by PCR and RFLP was done and showed that 10 of the 11 cases had an NDM plasmid that belonged to the same major cluster (pNDM-BC-5). Two ICU patients had NDM-1 producing K. pneumoniae in clinical isolates but the plasmid belonged to a different major cluster (pNDM-BC-7) and they were not considered part of the outbreak.

    All patients colonized or infected with NDM producing K. pneumoniae were placed on contact precautions in single rooms. All positive patients were cohorted to one side of the outbreak unit. Nursing staff were also cohorted into a clean cohort and an outbreak cohort. Weekly point prevalence screens were conducted.  Targeted surveillance of all admitted patients was started in March 2014 within all hospitals in the health authority. These combined measures resulted in halting transmission and the outbreak was declared over on March 10, 2014.


    Institutions that serve populations who frequently travel to regions where carbapenemase-producing organisms are endemic should have a robust surveillance and containment system for these organisms. Active surveillance and staff cohorting are important infection control measures. Molecular tools are helpful in understanding the patterns of transmission of these organisms and assisting with control measures.

    Manal Tadros, MBBS, MSc, PhD, FRCPC1,2, Linda MN Hoang, MD, FRCPC2,3, Matthew Croxen, PhD3, Michael Mulvey, PhD4,5, Benjamin Mack, MD, FRCPC1,2, Susan Roman, MD, FRCPC1, Joan Tomblin, MD, FRCPC1, Elizabeth Brodkin, MD1, Adriana Mendes, RN, BScN, CIC1, Ken Fakharuddin, Laboratory technician4, Suk Ching Wong, RN1 and Dale Purych, MD, FRCPC1,2, (1)Fraser Health Authority, Surrey, BC, Canada, (2)University of British Columbia, Vancouver, BC, Canada, (3)BCCDC Public Health Microbiology and Reference Laboratory, Provincial Health Services Authority, Vancouver, BC, Canada, (4)Nosocomial Infections, National Microbiology Laboratory, Winnipeg, MB, Canada, (5)University of Manitoba, Winnipeg, MB, Canada


    M. Tadros, None

    L. M. Hoang, None

    M. Croxen, None

    M. Mulvey, None

    B. Mack, None

    S. Roman, None

    J. Tomblin, None

    E. Brodkin, None

    A. Mendes, None

    K. Fakharuddin, None

    S. C. Wong, None

    D. Purych, None

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