1172. Next-Generation Sequencing for the Identification of Viruses in Pediatric Acute Encephalitis and Encephalopathy
Session: Poster Abstract Session: Clinical Infectious Diseases: CNS Infection
Friday, October 28, 2016
Room: Poster Hall
Posters
  • IDSA_2016_Kawada.pdf (1.0 MB)
  • Background: Acute encephalitis/encephalopathy is a severe neurological syndrome that is occasionally associated with viral infection. However, viral pathogens have rarely been identified because comprehensive detection assays have not been established. We evaluated the utility of next-generation sequencing (NGS) for detecting viruses in clinical samples of pediatric encephalitis/encephalopathy patients.

    Methods: We first determined the sensitivity and quantitative capability of NGS by comparing the NGS-determined number of sequences of human herpesvirus-6 (HHV-6) in clinical serum samples with the HHV-6 load measured using real-time PCR. HHV-6 was measured as it occasionally causes neurologic disorders in children. Next, we investigated the ability of NGS to detect viral sequences in 18 pediatric patients with acute encephalitis/encephalopathy of unknown etiology. DNA and RNA sequencing libraries were prepared using a Nextera XT and ScriptSeq v2, respectively. Indexed samples were pooled and sequenced on MiSeq with 2×75 bp or HiSeq 2500 with the 2×150 bp pair-end protocol to obtain an average of 8,674,293 reads.

    Results: The sensitivity of NGS for detection of HHV-6 sequences was equivalent to that of real-time PCR, and the number of HHV-6 reads was significantly correlated with HHV-6 load. In patients with acute encephalitis/encephalopathy, substantial reads of Coxsackievirus A9 and mumps viral sequences were detected in the cerebrospinal fluid of 2 and 1 patients, respectively. Additionally, Torque teno virus and Pepper mild mottle viral sequences were detected in the sera of one patient each.

    Conclusion: These data indicate that NGS may be useful for detection of causative viruses in patients with pediatric encephalitis/encephalopathy.

    Junichi Kawada, MD, Yusuke Okuno, MD, Yuka Torii, MD, Kazuhiro Horiba, MD, Takako Suzuki, MD, Shotaro Ando, MD, Yasuko Kamiya, MD and Yoshinori Ito, MD, Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Disclosures:

    J. Kawada, None

    Y. Okuno, None

    Y. Torii, None

    K. Horiba, None

    T. Suzuki, None

    S. Ando, None

    Y. Kamiya, None

    Y. Ito, None

    Findings in the abstracts are embargoed until 12:01 a.m. CDT, Wednesday Oct. 26th with the exception of research findings presented at the IDWeek press conferences.