1218. Evaluation of Ertapenem Efficacy in Sepsis of Rats induced by Cecal ligation and Puncture
Session: Poster Abstract Session: Clinical Infectious Diseases: Enteric Infections
Friday, October 28, 2016
Room: Poster Hall
Posters
  • IDSA poster pdf.pdf (319.3 kB)
  • Background: Intra-abdominal adhesions are still a major problem which are expected to be reduced by the provision of bacterial decontamination. Various antibiotics have been used to prevent the formation of adhesion in septic abdomen. The aim of this study is to demonstrate the efficacy of ertapenem in sepsis of rats induced by cecal ligation and puncture.

    Methods: Twenty eight male Wistar rats in weights ranging from 200 to 250 g, were used in this study. Rats were divided into 4 groups randomly. In all groups, bacterial peritonitis was created by cecal ligation and puncture method. Group 1 was considered as sham group. Groups 2, 3, and 4 were given, respectively, saline, a single dose of ertapenem and a dose of ertapenem intraperitoneally every day. Intra-abdominal adhesions were assessed 7 days after surgery by histopathological examination. Microbiological examination was performed through the ascites obtained.

    Results: Adhesion score decreased significantly by the application of ertapenem (p <0.001) and fibrosis scores were found to be significantly lower (p:0.005). Among all groups, the relationship between the decrease in the number of colonies and antibiotics application was not statistically significant (p:0.109). No statistically significant difference was found between the group given single dose of ertapenem and the group given multiple ertapenem (p:1).

    Conclusion: In the light of the data provided, peritoneal lavage with ertapenem appears to be effective in preventing the adhesion in septic rat abdomen. As no difference was detected at the end of a single dose and multiple dose administration of antibiotics in the adhesion scores, a single dose after surgery seems to be enough. It is thought that, the results need to be interpreted with a clinical study.

    Sukran Kose, Associate Professor1, Basak Gol Serin, Doctor1, Osman Yılmaz, Professor2, Mehmet Yıldırım, Associate Professor3, Ilkay Akbulut, Doctor1, Suheyla Serin Senger, Associate Professor1, Gulgun Akkoclu, Specialist1 and Gulden Diniz Unlu, Associate Professor4, (1)Clinic of Infectious Diseases and Clinical Microbiology, Izmir Tepecik Education and Research Hospital, Izmir, Turkey, (2)Laboratory Animal Science, Dokuz Eylul University, Izmir, Turkey, (3)Clinic of General Surgery, Izmir Bozyaka Education and Research Hospital, Izmir, Turkey, (4)Pathology, Izmir Tepecik Education and Research Hospital, Izmir, Turkey

    Disclosures:

    S. Kose, None

    B. Gol Serin, None

    O. Yılmaz, None

    M. Yıldırım, None

    I. Akbulut, None

    S. Serin Senger, None

    G. Akkoclu, None

    G. Diniz Unlu, None

    Findings in the abstracts are embargoed until 12:01 a.m. CDT, Wednesday Oct. 26th with the exception of research findings presented at the IDWeek press conferences.