1162. Clinical Usefulness of Real-Time Polymerase Chain Reaction for the Diagnosis of Vibrio vulnificus Infection Using Skin and Soft Tissues
Session: Poster Abstract Session: Clinical Infectious Diseases: Bone and Joint, Skin and Soft Tissue
Friday, October 28, 2016
Room: Poster Hall
Background: 

Vibrio vulnificus is a halophilic gram-negative bacillus isolated in seawater, fish, and shellfish. Infection by V. vulnificus is the most severe food-borne infection reported in the United States of America. Here, we aimed to examine the clinical usefulness of polymerase chain reaction (PCR) using tissue specimens other than blood samples as a diagnostic tool for V. vulnificus infection.

Methods: 

A retrospective study was conducted with patients who underwent real-time PCR of the toxR gene in both blood and skin tissues, including serum, bullae, swab, and operation room specimens between 2006 and 2009.

Results: 

The median V. vulnificus DNA load of 14 patients in real-time PCR analysis of serum at the time of admission was 638.5 copies/mL blood, which was within the interquartile range (IQR: 37–3225). In contrast, the median value by real-time PCR using the first tissue specimen at the time of admission was 16,650 copies/mL tissue fluid (IQR: 4419–832,500). This difference was statistically significant (p = 0.022). DNA copy numbers in tissues were less affected by short-term antibiotic administration than that in blood samples, and antibiotic administration increased the DNA copy number in some patients.

Conclusion: 

We found, for the first time, that DNA copy number in tissues of patients infected by V. vulnificus was higher than that in blood samples. Additionally, skin lesions were more useful than blood samples as specimens for PCR analysis in patients administered antibiotics for V. vulnificus infection before admission.

Dong-Min Kim, M.D., PhD., Internal Medicine, Chosun University Hospital, Gwangju, Korea, The Republic of, Na Ra Yun, M.D., PhD., Internal Medicine, Chosun University, School of Medicine, Gwangju, South Korea and Choon-Mee Kim, Ph.D, Premedical Science, College of Medicine, Chosun University, Gwangju, Korea, The Republic of

Disclosures:

D. M. Kim, None

N. R. Yun, None

C. M. Kim, None

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