593. Blood-borne Dengue Virus: A Case Report of Severe Dengue in a Hematopoietic Stem Cell Recipient
Session: Poster Abstract Session: Oh One World: Infections from Near and Far
Thursday, October 27, 2016
Room: Poster Hall
Background:

Dengue virus (DENV) transmission through blood products is rare and screening is not a standard practice in blood banks, even in endemic countries.

Methods:

We retrospectively report a case based on clinical data record and laboratory findings.

Results:

We report the case of a patient diagnosed with lymphoblastic lymphoma who developed severe dengue following autologous hematopoietic stem cell transplantation (HSCT). She presented with severe hemorrhagic events, shock, and serositis. Three days after platelet transfusion, the donor reported to the medical team with classical symptoms of dengue fever. Blood analyses were positive for IgM against DENV in the donor and recipient samples, with RT-PCR revealing 178,602 and 8,491 DENV RNA copies/ml, respectively. Transmission through platelet transfusion was confirmed by amplification and sequencing of the NS1 region of both viruses and comparisons with other samples, including samples from the same outbreak. Phylogenetic analysis demonstrated that viruses isolated from both the donor and HSCT recipient were identical in the sequenced region, supporting the hypothesis of blood component-transmitted dengue.

Conclusion:

Blood-borne DENV transmission may rarely result in severe case presentations. More studies are required to determine the incidence of DENV transmission by blood products and its relevance in public health. There is a substantial clinical need for new strategies for monitoring DENV transmission through blood transfusion during large outbreaks.

Maysa Alves, MD1, Vanessa Infante, MD2, Carolina Zenatti, MD2, Garles Vieira, MD2, Ivan Silva, PHD2, Sandra Kayano, MD2, Camila Romano, PHD3, Paulo Urbano, MSc3 and Esper Kallas, PHD4, (1)Infection Control, AC Camargo Cancer Center, São Paulo, Brazil, (2)AC Camargo Cancer Center, São Paulo, Brazil, (3)Virology laboratory, Tropical Medicine Institute, University of São Paulo, São Paulo, Brazil, (4)Department of Infectious and Parasitic Diseases, Faculty of Medicine, University of São Paulo, São Paulo, Brazil

Disclosures:

M. Alves, None

V. Infante, None

C. Zenatti, None

G. Vieira, None

I. Silva, None

S. Kayano, None

C. Romano, None

P. Urbano, None

E. Kallas, None

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