205. Detection of Borrelia in Early-Stage Lyme Disease Using T2 Magnetic Resonance
Session: Poster Abstract Session: Diagnostics: Bacteriology, Sequencing, and Resistance
Thursday, October 27, 2016
Room: Poster Hall
Background: Serological testing often fails to detect antibodies directed against Borrelia for patients with early-stage Lyme disease. By using direct detection of spirochaetemia, early stages of the disease may be diagnosed prior to seropositivity recognized by enzyme immunoassay (EIA) or western immunoblot (WB) tests. We have previously demonstrated a method of sensitive detection of Candida spp. using T2 magnetic resonance (T2MR), and in this study we apply this technique to the detection of Borrelia in blood.

Methods: B. burgdorferi, B. afzelii, or B. garinii were cultured in Barbour-Stoenner-Kelly media and cell counts were obtained by automated cell counting prior to spiking into blood. Samples (1.75mL) were processed by lysing red blood cells, concentrating target cells, and amplifying target cell DNA after cell disruption. Amplicons were hybridized to DNA-probe conjugated superparamagnetic particles. Presence of target amplicons were determined by the T2MR signal, which is ~10x higher for amplicon-bound and clustered particles than for amplicon-free and unclustered particles. Frozen samples (n=66) were obtained from cases of suspected Lyme disease characterized by symptoms, erythema migrans (EM), and results of EIA, WB, and/or PCR tests.

Results: Limits of detection (≥95% hit rate for n≥40 replicates) were 8 cells/mL for B. burgdorferi and B. garinii and 5 cells/mL for B. afzelii with no incidence of false positives. Among the 22 most probable clinical samples, classified by at least 2 positive EIA, WB, or PCR tests, 41% were positive for B. burgdorferi by T2MR. Five cases with EM and negative EIA or WB result were positive by T2MR. Two cases with EM and initial negative or equivocal EIA results that later seroconverted in a return visit were positive by T2MR. In 20 samples tested with PCR and T2MR, agreement was found on 1 positive and 12 negative samples. T2MR detected Borrelia in 7 samples reported negative by PCR.

Conclusion: Spiked blood mimicking spirochaetemia levels of ≥5-8 cells/mL were directly detected in blood using T2MR. B. burgdorferi cells were also detected in a subset of clinical samples from patients suspected of early-stage Lyme disease, including samples that would not have been diagnosed using conventional methods.

Jessica Snyder, PhD1, Cheryl Bandoski-Gralinski, MS1, Jessica Townsend, MS1, Heidi Giese, PhD1, Hans-Ulrich Thomann, PhD1, Robert Shivers, PhD1, Anna Schotthoefer, PhD2, Thomas Fritsche, MD, PhD3, Clayton Green, MD, PhD4, Steven Callister, PhD5, Lori Neely, Ph.D.1 and Tom Lowery, Ph. D.1, (1)T2 Biosystems Inc., Lexington, MA, (2)Marshfield Clinic Research Foundation, Marshfield, WI, (3)Marshfield Labs, Marshfield, WI, (4)Marshfield Clinic, Marshfield, WI, (5)Microbiology Research and Molecular Diagnostics Laboratories, Gundersen Health System, La Crosse, WI

Disclosures:

J. Snyder, T2 Biosystems: Employee and Shareholder , Salary

C. Bandoski-Gralinski, T2 Biosystems: Employee and Shareholder , Salary

J. Townsend, T2 Biosystems: Employee and Shareholder , Salary

H. Giese, T2Biosystems: Employee and Shareholder , Salary

H. U. Thomann, T2 Biosystems: Employee and Shareholder , Salary

R. Shivers, T2 Biosystems: Employee , Salary

A. Schotthoefer, T2 Biosystems: Research Contractor , Salary

T. Fritsche, None

C. Green, None

S. Callister, None

L. Neely, T2 Biosystems: Employee and Shareholder , Salary

T. Lowery, T2 Biosystems: Employee and Shareholder , Salary

Findings in the abstracts are embargoed until 12:01 a.m. CDT, Wednesday Oct. 26th with the exception of research findings presented at the IDWeek press conferences.