1401. Outbreak of Klebsiella pneumoniae Carbapenemase-Producing Providencia stuartii in an University Hospital in Brazil
Session: Poster Abstract Session: HAI: Outbreaks
Friday, October 28, 2016
Room: Poster Hall
Background: The emergence of multidrug resistance among gram negative microorganisms is a major public health problem. The characterization of bacterial strains can be of great assistance in the epidemiological investigation of nosocomial infections.

Methods: In the period of February to September 2014, 40 clinical samples of Providencia stuartii that showed resistance to carbapenems were isolated, when evaluated by Vitek2® automation system, from patients admitted to University Hospital of Londrina. The carbapenem resistance mechanism was evaluated by means of phenotypic tests, for the presence of carbapenemases: the modified Hodge test (MHT), addition of phenylboronic acid (PBA) and EDTA 100mM. Subsequently, the isolates were characterized genotypically by the PCR technique using specific primers for the genes blaKPCblaVIM e blaIMPblaOXA-48blaNDM and blaCTX-M. Molecular characterization was performed by ERIC-PCR technique.

Results: Of all isolates, 17 (42.5%) were isolated from urine, 8 (20%) from tracheal aspirates, 6 (15%) from blood, and 9 (22.5%) from secretion and tissues. All MHT presented dubious results, showing not be suitable for characterizing carbapenemases, especially for P. stuartii. All isolates were positive for PBA and negative for EDTA 100 mM. The PCR reaction for blaKPC gene was positive for all isolates, whereas the other genes showed negative results.

Conclusion: The molecular characterization showed the presence of a predominant clone. The worldwide spread of bacterial clones carrying resistance genes is a threat to bacterial infections control and antibiotic therapy. The Brazilian reality on the molecular epidemiology is still hazy. Most reports of β-lactamase-producing enterobacteria do not include information on plasmids and clones responsible for the spread of genes of β-lactams resistance.

Fabiana Hiromi Takashina, Resident, Talita Cristina Galvão, Resident, Luana Soares De Moraes, Resident, Amanda Cristina Ishioka De Oliveira, Pharmacy Graduated, Deise Moreira, Student of Pharmacy, Caroline Martins De Matos, Student of Pharmacy, Marcia Regina Eches Perugini, Professor of Microbiology at Londrina State University, Floristher Elaine Carrara Marroni, Professor of Microbiology at Londrina State University, Marsileni Pelisson, Professor of Microbiology at Londrina State University, Claudia Carrilho, MD, PhD and Eliana Carolina Vespero, Professor of Microbiology at Londrina State University, Londrina State University, Londrina, Brazil

Disclosures:

F. H. Takashina, None

T. C. Galvão, None

L. S. D. Moraes, None

A. C. I. D. Oliveira, None

D. Moreira, None

C. M. D. Matos, None

M. R. E. Perugini, None

F. E. C. Marroni, None

M. Pelisson, None

C. Carrilho, None

E. C. Vespero, None

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