1556. In Vitro Volatile Metabolite Signatures of Common Pathogenic Fusarium Species
Session: Poster Abstract Session: Mycology: Diagnostic
Friday, October 28, 2016
Room: Poster Hall

Background: Fusarium species cause severe, life-threatening infections in immunocompromised patients. Early diagnosis is critical due to the propensity of these species for adventitious sporulation and systemic dissemination, but difficult clinically due to the lack of specific, rapid, noninvasive diagnostic methods.

Methods: We examined the volatile organic metabolite profile of five common pathogenic Fusarium species in vitro, identifying key volatile organic metabolites (VOCs) using thermal desorption-gas chromatography (GC)/tandem mass spectrometry (MS), in parallel with GC-differential mobility spectrometry (DMS). DMS provides a rapid, portable method of identifying particular volatile metabolites in complex gas mixtures based on atmospheric pressure ionization followed by differential trajectories of these ion species across an asymmetric waveform electrical field.

Results: We identified a unique pattern of volatile secondary sesquiterpene metabolites for each Fusarium species by GC-MS, with corresponding identification of these species-specific metabolite signatures by GC-DMS. Fusarium solani (N = 5) emitted a signature of isocomene, farnesene, isocaryophyllene, and aciphyllene (Figure 1: left panel: representative GC-MS total ion chromatogram segment of relative abundance (total ion count) vs. retention time), right panel: representative GC-DMS plot of DMS metabolite intensity vs. VC (compensation voltage) vs. retention time). F. verticillioides (N = 4) produced cubenene, valencene, and acoradiene (Figure 2), F. proliferatum (N = 7) produced acora-3,7(14)-diene, himachalene, alloaromadendrene, and acoradiene (Figure 3), and F. oxysporum (N = 5) produced valencene and acoradiene.

Conclusion: Pathogenic Fusarium species produce unique secondary volatile metabolite signatures that are distinct from the volatile metabolite signatures of Aspergillus, Scedosporium/Lomentospora, and common pathogenic Mucorales. These fungal metabolite signatures may be used as targets for rapid, breath-based identification of Fusarium infections and allow differentiation from other invasive mold infections.

Figure 1. Fusarium solani.

Figure 2. Fusarium verticillioides.


Figure 3. Fusarium proliferatum.

Xinwei Yu, PhD1, Seena Koshy, PhD1, Obadah Aloum, MBBCh1, Lindsey R. Baden, MD1, Francisco M. Marty, MD, FIDSA2, Nathan Wiederhold, PharmD3 and Sophia Koo, MD1, (1)Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, (2)Division of Infectious Diseases, Brigham and Women's Hospital, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, (3)Univ. TX, San Antonio, TX

Disclosures:

X. Yu, None

S. Koshy, None

O. Aloum, None

L. R. Baden, None

F. M. Marty, Alexion: Scientific Advisor , Consulting fee
Ansun: Investigator , Research support
Astellas: Consultant and Investigator , Consulting fee and Research support
Basilea: Conference speaker , Speaker honorarium
Chimerix: Consultant and Investigator , Consulting fee and Research support
Gilead: Consultant and Investigator , Consulting fee and Research support
GlaxoSmithKline: Consultant and Investigator , Consulting fee and Research grant
LFB, S.A.: Consultant , Consulting fee
Merck: Consultant and Investigator , Consulting fee and Research support
Shire: Consultant and Investigator , Consulting fee and Research support
WHISCON: Investigator , Research support
Pfizer: Course speaker , Speaker honorarium
Fate Therapeutics: Scientific Advisor , Consulting fee

N. Wiederhold, BioMerieux: Grant Investigator , Research support

S. Koo, Wako Diagnostics: Grant Investigator , Research support

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