Methods: Serum samples were studied from 91 children 6-36 months old at healthy visits, during AOM, and after recovery 3 weeks later. Serum S100A12, IL-10, and ICAM-1 were measured by ELISA. Middle ear fluid permitted identification of otopathogens. A predictive model for infection, recovery, and causative otopathogen was developed.
Results: 225 samples were analyzed: 44 samples were collected at healthy visits when no otopathogen was detected, 41 at healthy visits when the child was asymptomatically colonized in the NP, but was not suffering an AOM, 90 at AOM visits and 50 at follow-Up visits. Seven possible combinations of biomarkers were considered in the analysis: S100A12 alone, IL-10 alone, ICAM-1 alone, S100A12 +IL-10, S100A12 + ICAM-1, IL-10 + ICAM-1 and S100A12 + IL-10 + ICAM-1. Child age and serum levels of S100A12, IL-10, and ICAM-1 were significantly predictive of AOM, recovery from AOM after treatment, and the specific causative pathogen.
A. Almudevar, None