1535. A pilot study to look at the effect of IL-28B polymorphism on IL-28 expression and immunological recovery among HIV infected individuals following ART
Session: Poster Abstract Session: HIV Pathogenesis and Reservoir and Cure
Friday, October 28, 2016
Room: Poster Hall
Posters
  • ID week poster- srinidhi final.pdf (862.4 kB)
  • Background: Single nucleotide polymorphism (SNP) of IL-28B at rs12979860 and rs8099917 influences the PEG-IFN treatment in HCV infection. Only few studies report effect of this polymorphism on immunological recovery among HIV-1 infected individuals on ART.

    Methods: Forty eight treatment naïve HIV infected individuals were recruited in this study and were followed up after 6-9 months of ART. Whole blood sample was collected before and after 6-9 months of ART. Absolute CD4+&CD8+ and CD4/CD8 ratio were estimated using BD FACS Count flow cytometer. IL-28B Polymorphism was detected by PCR-RFLP. Quantification of IL-28 mRNA expression and IL-28B plasma level were done by Real Time PCR and IL-28B ELISA respectively. HIV-1 viral load was measured using Abbott m2000rt. All the variables were analyzed using MedCalc statistical software.

    Results: There was a significant(p= <0.0001) increase in the absolute CD4+ T-cell count, CD4/CD3%, IL-28 mRNA following 6-9 months of ART.There was a significant (p = 0.03) association of CC genotype at rs12979860 with higher CD4+ T-cell count among treatment naïve HIV infected individuals. Immune Reconstitution Inflammatory Syndrome(IRIS) was reported in a patient with the genotype TT at rs12979860 and GG at rs8099917. There is no correlation of IL-28B polymorphism with IL-28mRNA and IL-28B plasma level among treatment naïve HIV infected individuals. Between HIV infected individuals and healthy controls significant difference in IL-28mRNA and no significant difference in IL-28 level was observed. There was positive correlation (p=<0.05,rho= 0.394) between IL-28mRNA and HIV-1 viral load in HIV infected individuals. In particular CT (p=0.0003,rho=0.867) at rs12979860 and GT(p=0.01,rho=0.810) at rs8099917 had a significant positive correlation.

    Conclusion: Our study showed significantly higher CD4+ T cells in HIV infected individuals with genotype CC prior to ART and significantly high CD4+ T cells and CD4/CD3% following ART. No association of IL-28B polymorphism with IL-28 mRNA and IL-28B plasma level was observed. The increase in IL-28 mRNA expression and HIV-1 viral load before ART and the significant increase in IL-28 mRNA levels after ART and the decrease in HIV viral load after ART in individuals with CT and GT genotypes is a mystery to be answered by further studies.

    Srinidhi BV, MD1, Sachithanandham J, PhD1, Pulimood SA, MD2, Abraham OC, MD3, Rupali P, MD3, Fletcher Gj, PhD1 and Kannangai R, MD, PhD1, (1)Clinical Virology, Christian Medical College, Vellore, India, (2)Dermatology, Christian Medical College, Vellore, India, (3)Internal Medicine, Christian Medical College, Vellore, India

    Disclosures:

    S. BV, None

    S. J, None

    P. SA, None

    A. OC, None

    R. P, None

    F. Gj, None

    K. R, None

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