1353. Colonization of Probiotics in Bangladeshi Infants after Different Administration Regimens
Session: Poster Abstract Session: Clinical Trials
Friday, October 28, 2016
Room: Poster Hall
Background: In healthy adults, colonization by probiotics is only transient, requiring daily or alternate daily dosing to maintain detectable levels. There is some evidence that probiotics can achieve longer term colonization in infants. We evaluated the duration of colonization by two probiotics in Bangladeshi infants.

Methods: Infants age 4 to 12 weeks (N=160) were randomized to one month of a combination of Lactobacillus reuteri DSM 17938 and Bifidobacterium longum infantis on three different schedules: daily, weekly, or biweekly (every two weeks) or to non-probiotic control and followed for two additional months. Stool samples were collected at baseline, daily for the first seven days after the first and last doses of probiotics and weekly throughout the study. The presence of probiotics in stool was determined using strain specific quantitative PCR assays. Levels of one of the two probiotic strains (Lactobacillus reuteriDSM 17938) in a preliminary subset of samples are presented here.

Results: On a subset of 47 infants (Daily Arm N=13, Weekly Arm N=11, Biweekly Arm N=11, Control Arm N=12) we quantified L. reuteri DSM 17938 levels at baseline, after 1 month of probiotic administration, and for seven days after the last dose of probiotic. The day of the last dose of probiotics we found an increase in probiotic quantity in the arms that received probiotics compared to control (+3452ng/ul increase compared to +2ng/ul) with the greatest increases in the Weekly (+9296ng/ul) and Daily (+1810ng/ul) arms. By seven days after probiotic administration, we found no differences between probiotic and control arms (Probiotic Arms = +259ng/ul, Control Arm = +213ng/ul).

Conclusion: One month of probiotic administration led to a transient increase in the level of one probiotic in stool; by one week post administration, however, there was no evidence of ongoing probiotic carriage, even after prior daily administration. The probiotic tested to date did not seem to have sustained colonization when given to infants with immature intestinal microbiota.

Yana Emmy Hoy-Schulz, PhD1, Kaniz Jannat, MBBS2, Thomas Roberts, BS3, Saira Zaidi, MPH3, Md. Mostafizur Rahman, BS2, Leanne Unicomb, PhD2, Stephen Luby, MD3 and Julie Parsonnet, MD, FIDSA4, (1)Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, School of Medicine, Stanford, CA, (2)Center for Communicable Diseases, International Center for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh, (3)Stanford University School of Medicine, Stanford, CA, (4)Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA

Disclosures:

Y. E. Hoy-Schulz, None

K. Jannat, None

T. Roberts, None

S. Zaidi, None

M. M. Rahman, None

L. Unicomb, None

S. Luby, None

J. Parsonnet, None

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