2092. Transfer of Bacteriophage MS2 versus Clostridium difficile Spores to Environmental Surfaces during Simulations of Patient Care
Session: Poster Abstract Session: Clostridium difficile: Outcomes, Testing, Prevention
Saturday, October 29, 2016
Room: Poster Hall
  • Microbial Transfer to Environmental Surfaces after Simulations of Patient Care.pdf (503.2 kB)
  • Background: Simulations can be valuable to understand dissemination of pathogens. Bacteriophage MS2 is a non-pathogenic virus commonly used to model spread of viruses in healthcare and non-healthcare settings. However, it is not known how well dissemination of MS2 correlates with dissemination of bacterial pathogens such as Clostridium difficile. Methods: During simulated patient care interactions, we evaluated environmental dissemination of bacteriophage MS2 and nontoxigenic Clostridium difficile spores inoculated onto a contaminated mannequin. After healthcare personnel performed a physical examination, surfaces (i.e. bedside table, side rail, stethoscope, IV pole, call button, curtains, trash can and floor) were sampled. We compared the C. difficile colony-forming units (CFU) and MS2 plaque-forming units (PFU) recovered from surfaces and frequency of contamination. Results: Sixty patient care simulations were performed by healthcare personnel. The concentration of MS2 and C. difficile on the mannequin decreased after 10 simulations, but the reduction was not statistically significant (MS2 4.3 vs 2.7 logs, C difficile 2.3 vs 1.8 logs; P>0.05 for both). Both pathogens disseminated to touched surfaces throughout the room (Figure 1). The average concentration of MS2 and C. difficile recovered from contaminated surfaces was similar (1.8 vs 1.1, P=0.01) and there was no difference in frequency of C. difficile and MS2 contamination (43% vs 33%, P=0.35). Conclusion: During simulations of patient care, dissemination of bacteriophage MS2 correlated well with dissemination of nontoxigenic C. difficile spores. Bacteriophage MS2 may be useful as a benign surrogate marker that can be applied in clinical care settings to investigate routes of dissemination of C. difficile and other pathogens. Figure 1. Concentrations of Bacteriophage MS2 and C. difficile on Surfaces