1284. Reduced numbers of T–cell, B-cell and CD4+ follicular helper T cell (Tfh) populations in infants with acute RSV infection
Session: Poster Abstract Session: Clinical Infectious Diseases: Respiratory Infections
Friday, October 28, 2016
Room: Poster Hall
Posters
  • Lymphopenia ID week_Final.pdf (1.5 MB)
  • Background: Our previous studies suggest that relative peripheral blood lymphopenia is associated with worse clinical outcomes in previously healthy children with acute RSV infection. Whether the lymphopenia is driven by a decreased in the T or B cell numbers has not been well characterized. We sought to characterize the T, including Tfh, and B cellular immune responses induced by RSV infection in previously healthy children representative of the whole disease spectrum.

    Methods: Previously healthy infants with RSV infection were enrolled and blood samples obtained at the time of evaluation in the Emergency Department (outpatients) or within 24 hours of hospitalization (inpatients). Healthy age-matched controls (HC) were also enrolled. Using the differences in T cell, B cell and CD4+ Tfh cell numbers and subsets according to disease severity defined as the need for hospitalization.

    Results: We enrolled a total of 64 children: 21 RSV+ outpatients, 11 RSV+ inpatients and 32 healthy age-matched controls. Overall, inpatients were younger than outpatients (median age: 2.2 vs 7.9 months; p<0.001). Total absolute numbers of CD3+, CD8+ and effector CD8+ T cells were reduced in both RSV+ inpatients and outpatients compared with HC (p<0.01), while total CD4+ and naïve CD4+ T-cells were decreased only in outpatients (p<0.01). On the other hand, absolute CD19+ B cell numbers and subsets were not significantly different between RSV groups, except for plasma B cells which were lower in inpatients vs. outpatients (p=0.03). Lastly, Tfh2 (CXCR3- CCR6), but not Tfh1 (CXCR3+CCR6-) or Th17 (CCR6+CXCR3-), were decreased only in patients hospitalized with RSV infection but not in outpatients (p=0.027).

    Conclusion: RSV infection was associated with reduced absolute numbers of total CD8+ T and CD8+ effector cells independent of disease severity, while the overall numbers of B-cells were minimally affected. Further studies are needed to understand the long-term impact of this dysfunctional immune response.

    Santiago Panesso, MD1, Victoria Best, PhD2, Raquel Giacomelli Cao, MD PhD3, Fang Ye, PhD2, Santtu Heinonen, MD, PhD4, Octavio Ramilo, MD, FPIDS5 and Asuncion Mejias, MD, PhD1, (1)Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital, Columbus, OH, (2)Center for Vaccines & Immunity, The Research Institute at Nationwide Children's Hospital, Columbus, OH, (3)Center for Vaccines & Immunity, Nationwide Children's Hospital, Columbus, OH, (4)Children's Hospital, Helsinki University Central Hospital, Helsinki, Finland, (5)Pediatrics, Section of Infectious Diseases, Nationwide Children's Hospital, Columbus, OH

    Disclosures:

    S. Panesso, None

    V. Best, None

    R. Giacomelli Cao, None

    F. Ye, None

    S. Heinonen, None

    O. Ramilo, Abbvie: Consultant and Scientific Advisor , Consulting fee and Speaker honorarium
    Medimmune: Scientific Advisor , Consulting fee
    Janssen: Consultant , Investigator and Scientific Advisor , Consulting fee and Research grant
    HuMabs: Scientific Advisor , Consulting fee
    Regeneron: Scientific Advisor , Consulting fee

    A. Mejias, Janssen: Grant Investigator and Scientific Advisor , Consulting fee and Research grant
    Alios: Consultant , Consulting fee
    Abbvie: CME lecture , Speaker honorarium
    Novartis: CME lecture , Speaker honorarium
    Gilead: Grant Investigator , Grant recipient

    Findings in the abstracts are embargoed until 12:01 a.m. CDT, Wednesday Oct. 26th with the exception of research findings presented at the IDWeek press conferences.