605. Experimental Zika Virus Infection in a Neotropical Primate Model
Session: Poster Abstract Session: Oh One World: Infections from Near and Far
Thursday, October 27, 2016
Room: Poster Hall
Posters
  • Vanchiere_ZIKA Poster_OCTOBER2016_IDWeek.pdf (430.3 kB)
  • Background: The epidemic of Zika virus (ZIKV) infection in South and Central America and its serious complications during pregnancy have highlighted the need for animal models of ZIKV disease for the study of prophylactic and therapeutic strategies prior to testing in humans. The purpose of this study was to determine whether inoculation of squirrel monkeys (Saimiri species) with Asian-lineage ZIKV results in infection similar to that seen in humans.

    Methods: Two Bolivian squirrel monkeys (Saimiri boliviensis boliviensis) and Two Guyanese squirrel monkeys (Saimiri sciureus) were inoculated in early pregnancy with 10e5 plaque-forming units (PFU) of ZIKV isolated from Mexico during the current epidemic. Blood, urine and saliva specimens were collected every other day for the first 14 days post-inoculation and then every 14 days thereafter for quantitative PCR assessment of Zika virus replication. Seroconversion was documented by plaque neutralization assay at 28 days post-inoculation.

    Results: All four animals remained clinically well after ZIKV inoculation and there were no abnormalities in the hematology and chemistry profiles performed at 5 days post-inoculation. Viremia was detected in two of the four animals inoculated with Zika virus, with peak viremia occurring at 5 and 9 days post-inoculation, followed by a rapid decline in detectable ZIKV. Viruria was detected at 9 and 14 days post-inoculation and salivary excretion was detected as late as 14 days post-inoculation. Three of the four ZIKV-inoculated animals had demonstrable neutralizing antibodies at 28 days post-inoculation.

    Conclusion:  This study provides evidence of the susceptibility of squirrel monkeys to infection with Asian-lineage ZIKV, supporting the hypothesis that these neotropical primates could serve as a reservoir and amplification host should a sylvatic cycle of ZIKV be established in South America.

    John Vanchiere, MD, PhD1, Julio C. Ruiz, DVM2, Nikos Vasilakis, PhD3, Thomas J. Kuehl, PhD4, Scott C. Weaver, PhD3 and Christian R. Abee, DVM2, (1)Pediatrics, LSUHSC-Shreveport, Shreveport, LA, (2)Veterinary Sciences, University of Texas MD Anderson Cancer Center, Bastrop, TX, (3)Pathology, University of Texas Medical Branch, Galveston, TX, (4)Obstetrics and Gynecology, Baylor-Scott & White Health, Temple, TX

    Disclosures:

    J. Vanchiere, None

    J. C. Ruiz, None

    N. Vasilakis, None

    T. J. Kuehl, None

    S. C. Weaver, None

    C. R. Abee, None

    Findings in the abstracts are embargoed until 12:01 a.m. CDT, Wednesday Oct. 26th with the exception of research findings presented at the IDWeek press conferences.