A blinded panel of 300 archived stool samples was prepared containing rotavirus, norovirus, group F adenovirus, astrovirus, and sapovirus, or none of these viruses. Three laboratories tested this panel by TAC, FGP or GPP (which only detects rotavirus, norovirus and group F adenovirus). Samples positive by RT-q PCR and sequencing were considered true positives and samples testing negative by all methods were considered true negatives.
Of 207 true positive samples, FGP detected 199 (96.1%) and TAC 174 (84.0%) samples. GPP detected 100 (78.7%) of the 127 samples positive for the three viruses in its platform. Detection rates for rotavirus were highest (100%, 89.5% and 95.8%, for FGP, TAC and GPP, respectively) followed by astrovirus (97.4%, 92.3%, n/a), norovirus (87.8%, 87.8% and 78.0%), sapovirus (97.5%, 75.6%, n/a) and adenovirus (97.3%, 73.6% and 57.8%). The sensitivity of FGP, TAC and GPP was highest for rotavirus (100%, 89.6%, and 95.8%, respectively) and lowest for adenovirus (97.4%, 70.0% and 57.9%). The specificity of the FGP ranged from 99.6% (sapovirus) - 95.6% (rotavirus), whereas TAC specificity ranged from 100% (rotavirus) - 98.9% (astrovirus) and GPP specificity ranged from 100% (rotavirus/ adenovirus) - 99.6% (norovirus). For specimens containing low viral load (Ct values > 31), FGP detected 59/64 (92.1%) samples followed by TAC (35/64 (54.6%)) and GPP (14/37 (37.8%)).
G. Weinberg, None
N. Halasa, Pfizer: Grant Investigator , Research grant
Biocryst: Grant Investigator , Research support
AstraZeneca: Grant Investigator , Research support
Baxter: Grant Investigator , Research grant
J. D. Chappell, None
F. Hassan, None
R. Selvarangan, None
L. Ward, None
M. D. Bowen, None
D. Payne, None
J. Vinjé, None