2184. Systemic Analysis of the mecA Gene Using a Bioinformatics Tool
Session: Poster Abstract Session: HAI: MRSA, MSSA, and Other Gram Positives
Saturday, October 7, 2017
Room: Poster Hall CD
  • 20170911_蔡欣怡_Systemic Analysis of the mecA Gene Using a Bioinformatics Tool.pdf (782.6 kB)
  • Background:

    The mecA gene, carried by methicillin-resistant Staphylococcus aureus (MRSA), allows the bacterium to promotes bacterial resistance to antibiotics such as methicillin, penicillin, and other penicillin-like antibiotics. Our objectives are to use a bioinformatics tool to analyze the sequence of the mecA gene, which is spread on the SCCmec genetic element, and to investigate the relationship between each mecA gene.


    From 2008 to 2016, we collected 229 MRSA from bacteremia; we extracted DNA from the MRSA and designed specific primers to target mecA using PCR. The primer used are listed in mec A-1( 5'-GGGATCATAGCGTCATTATTC-3') and mec A-2( 5'-AACGATTGTGACACGATAGCC-3'). We determined whether the mecA gene was present by using electrophoresis and then sequenced the MRSA samples in which it was present. The POWER tool was employed to analyze the mecA gene and compile a pedigree chart.


    Using the sequencing data, we created an MRSA database, and the BLAST findings demonstrated that most of the mecA genes were similar, with over 95% identified. The pedigree chart illustrates that there are four groups of mecA genes, and these groups were found to be not differentiated between the sources of the MRSA, whether from communities or hospital association infections.


    Our findings indicate that even though there were four groups with ancestors in the pedigree chart, no significant difference was found between MRSA from community- and hospital-associated infections. We plan to collect more MRSA samples for analysis and investigate the differences between MRSA groups and MRSA from various geographical regions.

    Shin-Yi Tsai, MD1,2,3, Fu-Chieh Chang, MT4, Kevin Sheng-Kai Ma, MD1, Cheng-Wei Hsu, MD1, Po-Ya Tung, MD1,3, Yan-Jiun Hung, MD1,3, Yi-Ting Chou, MT1 and Chien-Feng Kuo, MD5, (1)Department of Laboratory Medicine, Mackay Memorial Hospital, Taipei, Taiwan, (2)Johns Hopkins University, Baltimore, MD, (3)Department of Medicine, Mackay Medical College, New Taipei City, Taiwan, (4)Infection control center, Mackay Memorial Hospital, Taipei, Taiwan, (5)Department of Infectious Disease, Mackay Memorial Hospital, Taipei, Taiwan


    S. Y. Tsai, None

    F. C. Chang, None

    K. S. K. Ma, None

    C. W. Hsu, None

    P. Y. Tung, None

    Y. J. Hung, None

    Y. T. Chou, None

    C. F. Kuo, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 4th with the exception of research findings presented at the IDWeek press conferences.