1242. Quantitative Analysis of Microbial Burden on LTCF Environmental Surfaces
Session: Poster Abstract Session: Healthcare Epidemiology: Non-acute Care Settings
Friday, October 5, 2018
Room: S Poster Hall
Posters
  • ID WEEK LTC.pdf (538.8 kB)
  • Background:

    There is a lack of data on environmental surface contamination in long-term care facilities (LTCF), despite multiple reports of outbreaks of multi-drug resistant organisms in these settings.  Therefore, we conducted a quantitative analysis of the microbial burden and prevalence of epidemiologically important pathogens (EIP) found on LTCF environmental surfaces. 

     

    Methods:

    Microbiological samples were collected using Rodac plates from resident rooms and common areas in five LTCFs. At each facility, five samples from up to ten different available environmental surfaces were collected from a room of a resident reported to be colonized with EIP, as well as from a room of a resident reported to be non-colonized.  In addition, five samples from up to ten different environmental surfaces were collected from two common areas in the facility. EIPs were defined as MRSA, VRE, C. difficile and multi-drug resistant Gram negative bacilli.  Data were analyzed for each environmental site sampled in a resident room or common area based on total bacterial colony forming units (CFU), mean CFU per Rodac, total EIP by site and mean EIP counts per Rodac.

     

    Results:

    The below table summarizes total EIP recovered from environmental sites by reported EIP colonization status of the resident. Rooms of residents with reported colonization had much greater EIP counts per Rodac (8.32, 95%CI 8.05, 8.60) than rooms of non-colonized residents (0.78, 95% CI 0.70, 0.86).  MRSA was the most common EIP recovered from Rodacs, followed by C. difficile. Very few EIPs were recovered from the common areas sampled at these LTCFs.

     

    Conclusion:

    We found varying levels of CFU and EIP on environmental sites at LTCFs.  Colonization status of a resident was a strong predictor of higher levels of EIP being recovered from his/her room.


    Table: Total EIP recovered from environmental sites in residential rooms

    Site

    Number of Rodac Samples

    Total EIP by Site

    Mean EIP Counts per Rodac

    Number of Rodac Samples

    Total EIP by Site

    Mean EIP Counts per Rodac

     

    Non-Colonized Resident Rooms

    Colonized Resident Rooms

    Bathroom Floor

    54

    35

    0.65

    55

    1820

    33.09

    Bed Rail

    48

    20

    0.42

    45

    614

    13.64

    Over Bed Table

    48

    24

    0.50

    55

    123

    2.24

    Nightstand

    55

    1

    0.02

    49

    223

    4.55

    Sink

    55

    251

    4.56

    49

    371

    7.57

    Chair

    35

    1

    0.03

    44

    361

    8.20

    Overall Sites Sampled

    433

    337

    0.78

    428

    3561

    8.32

     

    Lauren DiBiase, MS1, Amy Powell, MPH2, Maria Gergen, MT (ASCP)1, David J. Weber, MD, MPH1,3, Emily E. Sickbert-Bennett, PhD, MS4 and William A. Rutala, PhD, MPH5, (1)Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, NC, (2)Division of Infectious Diseases, UNC School of Medicine, Chapel Hill, NC, (3)Medicine, Pediatrics, Epidemiology, University of North Carolina, School of Public Health, Chapel Hill, NC, (4)Department of Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, NC, (5)Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, NC

    Disclosures:

    L. DiBiase, None

    A. Powell, None

    M. Gergen, None

    D. J. Weber, None

    E. E. Sickbert-Bennett, None

    W. A. Rutala, None

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