2424. Review of Antimicrobial Susceptibility Profile of Different Nocardia Species, a Tertiary Center Experience
Session: Poster Abstract Session: Treatment of AMR Infections
Saturday, October 6, 2018
Room: S Poster Hall
Posters
  • Review of Antimicrobial Susceptibility Profile of Different Nocardia_NW edits.pdf (203.2 kB)
  • Background:

    Nocardia spp. are ubiquitous Gram-positive weakly acid-fast environmental microorganisms.  Although considered an opportunistic infection, approximately 1/3 of the reported infections are in immunocompetent patients. Treatment is usually challenging, prolonged and involves multiple agents depending on the site of infection, clinical syndrome and the immune status of the patient.

    Methods:

    We conducted a retrospective review of clinical samples with positive cultures for Nocardia spp. from 2011-2017.

    Specimens were cultured in MGIT broth or on Middlebrook agar biplates and isolated colony growth was then identified using MALDI-TOF MS or 16S rDNA gene sequencing. Antimicrobial susceptibility testing was performed using the TREK Sensititre Rapid Growing Mycobacteria Plate.

    Results:

    We reviewed total of 1840 samples positive for Nocardia spp. Most commonly isolated species included N. cyriacigeorgica (16.9%), N. nova complex (15.7%), N.farcinica complex (14.8%), N. brasiliensis (11.5%) and N. abscessus complex (8.2%). Susceptibilities of the more common Nocardia species are shown in the graph.

    Source of the positive cultures was variable with majority (>60%) from pulmonary source (sputum, BAL and lung tissue), blood in 5.7% and brain in 3.6%.

    Most common Nocardia species isolated from brain specimens were N. farcinica complex (24/59) followed by N. abscessus complex (17/59). Most common Nocardia species isolated from blood were N. farcinica complex (38/99) followed by N. nova complex (22/99) and N. cyriacigeorgica (15/99).

    Conclusion:

    The antimicrobials that continue to show high activity against most Nocardia species (>95%) are: amikacin, linezolid and TMP/SMX.

    N. pseudobrasiliensis was noted to have high rates of resistance to TMP/SMX (87%).

    N. farcinia, N. brasiliensis and N. transvalensis/wallacei complex were >90% susceptible to amoxicillin/clavulanate. Clarithromycin had >99% activity against N. nova complex while both ceftriaxone and doxycycline had> 90% activity against N. abscessus complex.

    It is crucial to identify Nocardia species and obtain susceptibilities to help better choose the regimen with the best clinical outcome.

    Ahmed Hamdi, MD1, Omar Abu Saleh, MD2, Madiha Fida, MD1, Alexandra Bryson, PhD3 and Nancy L. Wengenack, PhD, FIDSA4, (1)Infectious Disease, Mayo Clinic, Rochester, MN, (2)Division of Infectious Diseases, Mayo Clinic, Rochester, MN, (3)Mayo Clinc, Rochester, MN, (4)Division of Clinical Microbiology, Mayo Clinic, Rochester, MN

    Disclosures:

    A. Hamdi, None

    O. Abu Saleh, None

    M. Fida, None

    A. Bryson, None

    N. L. Wengenack, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 3rd with the exception of research findings presented at the IDWeek press conferences.