2090. Decontamination of Fusarium oxysporon from a Central Line Needleless Access Device using a 70% Isopropyl Alcohol Impregnated Port Protector
Session: Poster Abstract Session: Healthcare Epidemiology: Device-associated HAIs
Saturday, October 6, 2018
Room: S Poster Hall
  • ID WEEK 2018 POSTER 2090_Corrected.pdf (1.7 MB)
  • Background: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFU’s) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa & C. glabrata. The effect against F. oxysporon has not been reported.

    Figure 1. Port protector covering needleless access device

    Methods: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 X 106 CFU were loaded to the surface of 8 NAD’s and allowed to air dry, and (2) the surface of 20 NAD’s were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NAD’s were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NAD’s were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days.

    Results: In all cases, NAD’s that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NAD’s lacking the decontamination step invariably showed abundant growth.

    Figure 2. Growth in broth after 7 days of decontaminated (+) vs non-decontaminated  (-) NAD’s, with appropriate controls

    Figure 3. Growth on plates after 7 days of decontaminated (+) vs non-decontaminated  (-) NAD’s, with appropriate controls. Ten replicates performed.

    Conclusion: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices.


    Julianne Green, MD, PhD1, Alan Junkins, Ph.D.2, Kristina Bryant, MD1, Gordon Stout, BS1 and Charles Woods, MD, MS, FIDSA, FSHEA, FPIDS1, (1)Pediatrics, University of Louisville School of Medicine, Louisville, KY, (2)Norton Healthcare, Norton Hospital, Louisville, KY


    J. Green, None

    A. Junkins, None

    K. Bryant, None

    G. Stout, None

    C. Woods, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 3rd with the exception of research findings presented at the IDWeek press conferences.