Background: Fusarium species, pervasive environmental fungi, cause disseminated infection in immunocompromised hosts including central line associated bloodstream infections (CLABSIs), with a 75% mortality rate. Many hospitals utilize 70% isopropyl alcohol impregnated port protectors over needleless access devices (NADs) to reduce CLABSIs (Figure 1). These port protectors achieve ≥4-log reduction in colony-forming units (CFUs) of S. aureus, S. epidermidis, E. coli, C. albicans, P. aeruginosa & C. glabrata. The effect against F. oxysporon has not been reported.
Figure 1. Port protector covering needleless access device
Methods: F. oxysporon was grown on Sabouraud Dextrose (Sab Dex) agar. Two protocols were used: (1) aliquots of ~1 X 106 CFU were loaded to the surface of 8 NADs and allowed to air dry, and (2) the surface of 20 NADs were contaminated via touching to a dense lawn of Fusarium on culture plates. Half of the NADs were decontaminated with a port protector for one minute; the other half had no decontamination step (along with positive/negative controls). NADs were then (1) placed whole in Sab Dex broth or (2) touched to a fresh Sab Dex agar plate, and growth observed for 7 days.
Results: In all cases, NADs that had been decontaminated with the alcohol-impregnated port protector showed no growth after seven days in broth (Figure 2) or on plates (Figure 3). NADs lacking the decontamination step invariably showed abundant growth.
Figure 2. Growth in broth after 7 days of decontaminated (+) vs non-decontaminated (-) NADs, with appropriate controls
Figure 3. Growth on plates after 7 days of decontaminated (+) vs non-decontaminated (-) NADs, with appropriate controls. Ten replicates performed.
Conclusion: Use of two different techniques demonstrates that a 70% isopropyl alcohol impregnated port protector achieves decontamination of F. oxysporon from the surface of needleless access devices.
K. Bryant, None
G. Stout, None
C. Woods, None