2315. Neisseria meningitidis oro-pharyngeal carriage, serogroups and clonal complex in children and adolescents in Argentina
Session: Poster Abstract Session: Pediatric Bacterial Infections
Saturday, October 6, 2018
Room: S Poster Hall
Posters
  • Meningo IDWeek 26 09 18 version final.pdf (694.4 kB)
  • Background:

    Neisseria meningitidis (Nm) pharyngeal carriage is a necessary condition for invasive meningococcal disease. In 2017, Argentina introduced a tetravalent meningococcal conjugated vaccine (MenACYW) to the National Immunization Program for children. We present the first carriage study in children in the prevaccine era. Aims: 1) to assess the rate of Nm carriage in healthy children and adolescents attending a public hospital in Buenos Aires city; 2) to determine serogroup and clonal complex distribution; 3)to determine carriage risk factors by age.

    Methods:

    Cross-sectional study including children 1-17 yrs, stratified in two age groups (1-9 yrs and 10-17 yrs) assisted at Ricardo Gutiérrez Children Hospital between March-December 2017. Oro-pharyngeal swabs were plated and meningococci identified by conventional microbiology methods. Serogroup was determined by PCR. Clonal complex was determined by MLST.

    Results:

    A total of 1751 children were included. Group aged 1-9 yrs: 38 Nm were isolated from 943 samples collected: overall carriage 4.0%. Serogroups distribution: B 26.3%, Y 2.6%, W 5.3%, Z 5.3%, non-groupable 7.9% and non-capsulated 52.6%. Clonal complex was determined for 25 isolates. Attendance at social venues was the only independent predictor of Nm carriage (adjusted OR:2.02, CI95%=1.01-4.03;p=0.04).

    Group aged 10-17 yrs: 76 Nm were isolated from 808 samples: overall carriage 9.4%. Serogroups distribution: B 19.7%, C 5.3%, W 7.9%, Y 9.2%, Z 5.3%, non-groupable 7.9% and non-capsulated 44.7%. Clonal complex was determined for 58 isolates. Independent predictor of Nm carriage: attendance at night clubs (adjusted OR:3.38, CI95%=1.28-8.93;p=0.013); passive smoking at home (adjusted OR:0.55, CI95%=0.32-0.93;p=0.025). Preliminary data show a total of 10 different clonal complexes present among all serogroups in the 1-9 yrs group aged and 13 in 10-17yrs.

    Conclusion:

    Overall carriage was higher in the 10-17 yrs population. The non-encapsulated Nm was prevalent in both groups and serogroup B was the most frequent among the encapsulated. The results demonstrated a high diversity of N. meningitis in pharyngeal carriage.

    Angela Gentile, MD1, Maria Paula Della Latta, MD2, Barbara Wisner, Biochemist3, Mercedes Bloch, MD2, Luisina Martorelli, PhD3, Cecilia Sorhouet Pereira, Biochemist3, Mabel Regueira, MD4, Maria Del Valle Juarez, MD2, Veronica Umido, MD5 and Adriana Efron, Biochemist3, (1)Epidemiology, Hospital de Niños "Ricardo Gutiérrez", Buenos Aires, Argentina, (2)Epidemiology Department, Hospital de Niños "Ricardo Gutiérrez", Buenos Aires, Argentina, Buenos Aires, Argentina, (3)INEI-ANLIS 'Dr. Carlos G. Malbrán", Buenos Aires, Argentina, Buenos Aires, Argentina, (4)Servicio Bacteriologia Clinica, Instituto Nacional de Enfermedades Infecciosas ANLIS "Dr. Carlos G Malbran", Buenos Aires, Argentina, (5)Epidemiology, HOSPITAL DE NIÑOS RICARDO GUTIERREZ, Buenos Aires, Argentina

    Disclosures:

    A. Gentile, None

    M. P. Della Latta, None

    B. Wisner, None

    M. Bloch, None

    L. Martorelli, None

    C. Sorhouet Pereira, None

    M. Regueira, None

    M. D. V. Juarez, None

    V. Umido, None

    A. Efron, None

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 3rd with the exception of research findings presented at the IDWeek press conferences.