36. Molecular and clinical epidemiology of carbapenem-resistant Enterobacter aerogenes strains isolated from a tertiary hospital in China
Session: Posters in the Park: Posters in the Park
Wednesday, October 3, 2018: 5:30 PM
Room: N Hall D Opening Reception and Posters in the Park Area
  • idweek poster-v1.pdf (393.4 kB)
  • Background: The more and more frequently reported carbapenem-resistant Enterobacteriaceas called for alarm worldwide. In this study, the resistance mechanism and epidemiology of carbapenem-resistant Enterobacter aerogenes (CREA) which isolated in a Chinese tertiary hospital were investigated.

    Methods: Antimicrobial susceptibility testing of 17 antibiotics were performed. b-lactamase genes were detected. Efflux pump phenotype test and outer membrane porin SDS-PAGE were performed. Clonal relationship was investigated by PFGE (pulsed field gel electrophoresis). S1 nuclease-PFGE, Restriction Fragment Length Polymorphism(RFLP) analysis and plasmid incompatibility groups analysis were performed to analyze blaKPC-2 carrying plasmids. Epidemiological data were collected via history review.

    Results: Only tigecycline and colistin maintained high level susceptibility to 14 CREA isolates. Five distinct groups (PFGE types A–E) were observed. All 14 isolates carried the blaKPC-2. Only one isolates lost Omp36 porin. Efflux pump had no contribution to these 14 isolates. S1 nuclease-PFGE indicated that the size of blaKPC-2 carrying plasmids were among 20kb to 200kb. RFLP analysis divided blaKPC-2 carrying plasmids into 7 groups. Plasmids can be divided into 5 incompatibility groups. Epidemiological and molecular investigations showed that PFGE type A group, including 11 KPC-2-producing clinical isolates, was primarily responsible for the dissemination.

    Conclusion: Our findings suggest further studies should focus on judicious use of available antibiotics and strict implementation of infection-control measures to avoid the rapid spread or clonal dissemination caused by CREA in healthcare facilities

    Min Hao, MD and Xiaohua Qin, MD, Huashan Hospital, Shanghai, China


    M. Hao, None

    X. Qin, None

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