Methods: As a way to help facilitate WGS implementation, we sought to identify the challenges for the establishment and use of CLIA-compliant WGS workflows in SPHLs. An environmental scan was performed in which we assessed materials produced at CDC, by the Association of Public Health Laboratories (APHL), by a Next Generation Sequencing Tri-agency workgroup, as well as published papers and guidance. We also engaged stakeholders through conversations with SPHL partners, APHL, and several groups within CDC.
Results: Our analysis revealed relevant resources and key WGS validation materials were dispersed and difficult to locate. To address this, we developed a CDC Next-generation Sequencing Resource Roadmap, to house key materials. After we reviewed, selected, and collated the resources, our web developer created a visual roadmap webpage to guide the user through the resources. This roadmap was then reviewed and tested for initial use internally at CDC.
Conclusion: This communication tool has the potential to provide critical resources needed to develop functional WGS validation strategies.
R. Lindsey, None
A. Mercante, None
A. Raziano, None
L. Draper, None