The bacterial colonization of urinary catheters is a major source of hospital acquired urinary tract infections (HAUTI). Bacteria repellent coatings could lower HAUTI prevalence and minimize antimicrobial usage. We report a model of spontaneous bacterial colonization of an intravesical catheter in a spinalized rat model, and the first in vivo proof of the efficacy of Lubricant-Infused Catheters (LICs) in preventing bacterial colonization.
LICs preparation: Oxygen plasma treated polyethylene catheters were immediately placed in a vacuum desiccator and 200 uL of trichloro (1H,1H,2H,2H-perfluorooctyl) silane) was placed beside the catheter segments. The vacuum pump connected to the desiccator was turned on with the exit valve closed once a pressure of -0.08 MPa was achieved. The chemical vapour deposition process was initiated for 4 hours. Catheters were removed from the desiccator and placed in an oven at 60oC for a minimum of 12 h in order to complete the modification process. Catheters were saturated with a biocompatible fluorocarbon-based lubricant (perfluorodecalin) prior to implantation.
centimeters long native catheters and LICs were surgically implanted in the bladder of rats
spinalized 19 days prior and programmed to undergo cystometry experiments 48 hours later. Each rat was maintained
individually in a cage with food and water ad libitum until bladder functional
evaluation, and benefitted from a of trimethoprim
sulfadoxine and fluoroquinolone
prophylaxis. At the
end of the cystometry experiments, the animals were euthanized
and the bladder catheter was removed. A 1cm section from the intravesical end of the catheter was cut and placed in 1 ml
of Amies medium (eSawb, Copan), vortexed and sonicated. 10 µl of the suspension was plated on URIselect4
medium (Bio-Rad, Hercules, Ca) for bacterial
A significant reduction in bacterial colonization with Enterobacteriaceae and Enterococci was observed in the LICs group (N=6, below 100 CFU threshold) compared to the native catheter group (N=5, average 8200 CFU) (p<0.02).
Lubricant-infused catheters effectively prevent bacterial colonization in vivo and provide an attractive and non-selective option for HAUTI prevention.
J. Faugeroux, None
D. Behr-Roussel, None
M. Rottman, None
T. Didar, None