1053. Biofilm production and clinical characteristics of S. maltophilia causing persistent or relapsing bacteremia
Session: Poster Abstract Session: Bacteremia and Endocarditis
Friday, October 5, 2018
Room: S Poster Hall
Background:

This study aimed to identify clinical or microbiological factors related to persistence or recurrence of Stenotrophomonas maltophilia bacteremia in adult patients.

Methods: S. maltophilia isolated from blood in 2 tertiary hospitals between 2011 to 2017 were investigated. Persistent bacteremia was defined as the consecutive blood culture positive for ≥ 5 days after initiation of appropriate antibiotics therapy. Relapse was defined as isolation of S. maltophilia from blood after completion of antibiotics treatment for the first episode of bacteremia. Biofilm formation was assessed in 96-well polystyrene plate with Trypticase Soy Broth using 0.5% crystal violet staining. The presence of smf-1 gene was detected by polymerase chain reaction.

Results: Of total 100 patients with S. maltophilia bacteremia, 10 of persistent, 8 of relapsing and 46 of non-persistent, non-relapsing cases were investigated. The presence of indwelling urinary catheter (p=0.011), nasogastric tube (p=0.003), mechanical ventilator treatment (p=0.001), previous colonization of S. maltophilia (p=0.016) were more frequently observed in patients with persistent bacteremia compared to non-persistent, non-relapsing bacteremia cases. In patients with relapsing bacteremia, hematologic malignancy (p=0.022), neutropenia (p=0.001), and concomitant isolation of S. maltophilia in clinical samples other than blood (p=0.041) were more common than non-persistent, non-relapsing bacteremia patients. Catheter related infection (37.0%) followed by pneumonia (28.3%) was the most common primary focus of non-persistent, non-relapsing bacteremia whereas pneumonia was the most frequent cause of bacteremia in both of persistent and relapsing cases (40.0% and 50.0%). Most of isolates (63 of 64) were susceptible to cotrimoxazole. The resistance to levofloxacin were comparable among isolates from persistent, relapsing and non-persistent, non-relapsing cases (10.0% vs. 12.5% vs. 15.2%, p= 0.988). Biofilm formation ability was not significantly different between three groups (optical density at 595, mean ± SD, 0.69 ± 0.34 vs. 0.78 ± 0.33 vs. 0.70 ± 0.33, p= 0.529). The smf-1 gene was found in all isolates.

Conclusion: More careful treatment approaches to patients with risk factors for S.maltophilia treatment failure should be warranted.

Seung Ji Kang, MD1, Tae Hoon Oh, MD1, Younggon Jung, M.D.2, Seong Eun Kim, MD1, Uh Jin Kim, MD1, Hee-Chang Jang, MD1, Kyung-Hwa Park, MD1 and Sook-In Jung, MD1, (1)Chonnam National University Medical School and Hospital, Gwangju, Korea, Republic of (South), (2)Chonnam National University Hospital, Gwang ju, Korea, Republic of (South)

Disclosures:

S. J. Kang, None

T. H. Oh, None

Y. Jung, None

S. E. Kim, None

U. J. Kim, None

H. C. Jang, None

K. H. Park, None

S. I. Jung, None

Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 3rd with the exception of research findings presented at the IDWeek press conferences.