2492. Clinical, virologic, and immunologic characteristics of Zika virus infection in a cohort of US patients.
Session: Poster Abstract Session: Virology Potpourri
Saturday, October 6, 2018
Room: S Poster Hall
Posters
  • POSTER IDWEEK.pdf (891.9 kB)
  • Background:  The clinical, virologic and immunologic characteristics of Zika virus (ZIKV) infections in US patients are poorly defined.

    Methods:  US patients with suspected Zika virus (ZIKV) infection were enrolled and clinical data and specimens were prospectively collected. Body fluids were tested for ZIKV RNA by PCR and blood was tested using serologic and cellular immune assays. Findings from those with confirmed ZIKV infections (cases) and ZIKV-negative controls were compared.

    Results:   We enrolled 45 cases and 14 controls. The most commonly reported symptoms among cases and controls were maculopapular rash (97.8% and 81.8%), fatigue (86.7% and 81.8%) and arthralgia (82.2% and 54.5%), respectively. The sensitivity and duration of detection by PCR were highest in whole blood samples (94% of 35 cases who had samples collected up to day 79 post illness onset were positive); strikingly, 84% of those were still positive at 65-79 days post illness onset (Fig 1). ZIKV neutralizing antibodies were detected in all cases and none of the controls, and titers were significantly higher in dengue virus (DENV)-experienced subjects than in DENV-naïve ones (Fig 2).  Among cases, anti-ZIKV IgG antibodies were also significantly higher in DENV-experienced patients, while anti-ZIKV IgM antibodies were no higher in DENV-experienced compared to naïve ones.  Using intracellular cytokine staining, the highest frequencies of T cells producing IFN-γ, IL-2 and/or TNF-α were against the NS1, NS3, and NS5 proteins for CD4+ T cells, and against the E, NS3, and NS5 proteins for CD8+ T cells (Fig 3).

    Conclusion:  Detection of ZIKV RNA was more frequent and much more prolonged in whole blood samples compared to other body fluids. Diagnostic molecular assays on this easily obtained fluid should be prioritized for point-of-care development. Robust cellular responses to E, NS3 and NS5 proteins could have implications for vaccine development.

    Hana M. El Sahly, MD, FIDSA1, Rodion Gorchakov, MS2, Kristy Murray, DVM, PhD3, Shital Patel, MD4, Robert L. Atmar, MD5, Wendy Keitel, MD, FIDSA6, Daniel Hoft, MD, PhD7, Jill Barrett, MPH8, Jason Bailey, PhD9, Nadine Rouphael, MD, FIDSA10, Srilatha Edupuganti, MD11, Vanessa Raabe, MD10, Henry Wu, MD10, Jessica Fairley, MD10, Muktha Natrajan, PhD12, Lilin Lai, MD13 and Mark J. Mulligan, MD, FIDSA13, (1)Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, (2)Pediatrics/Tropical Medicine, Texas Children Hospital/ Baylor College of Medicine, Houston, TX, (3)Pediatrics-Tropical Medicine, Texas Children Hospital, Baylor College of Medicine, Houston, TX, (4)Baylor College of Medicine, Dept. of Medicine, Houston, TX, (5)Medicine, Baylor College of Medicine, Houston, TX, (6)Molecular Virology & Microbiology and Medicine, Baylor College of Medicine, Houston, TX, (7)Saint Louis University Health Sciences Center, St. Louis, MO, (8)The EMMES Corp, Rockville, MD, (9)Emmes Corporation, Rockville, MD, (10)Emory University, Atlanta, GA, (11)Medicine/Infectious Diseases, Emory University School of Medicine, Decatur, GA, (12)Emory University Hope Clinic, Atlanta, GA, (13)Hope Clinic-Emory University, Decatur, GA

    Disclosures:

    H. M. El Sahly, None

    R. Gorchakov, None

    K. Murray, None

    S. Patel, None

    R. L. Atmar, Takeda Vaccines, Inc.: Investigator , Research grant .

    W. Keitel, None

    D. Hoft, None

    J. Barrett, None

    J. Bailey, None

    N. Rouphael, None

    S. Edupuganti, None

    V. Raabe, None

    H. Wu, None

    J. Fairley, None

    M. Natrajan, None

    L. Lai, None

    M. J. Mulligan, None

    See more of: Virology Potpourri
    See more of: Poster Abstract Session

    Findings in the abstracts are embargoed until 12:01 a.m. PDT, Wednesday Oct. 3rd with the exception of research findings presented at the IDWeek press conferences.