Methods: Pieces (7 mm in length) of RenaSil Silicone Tubing were placed in the bladders of BALB/c female mice (n=5/group). Approximately 1 x 108 colony-forming units/mL ESKAPE pathogens (50 µl) were inoculated into the bladder, and animals were returned to cages for 18 h. One hundred microliters of 2.5% GML Gel (50:50 mix of saline and human-approved glycols) or phosphate-buffered saline (placebo) was administered into the bladders. After 2 h, animals were euthanized and colony-forming units in bladders and on catheters were determined, and histological analysis of bladders was performed.
Results: GML Gel was bactericidal against ESKAPE pathogens at 2 h post-treatment. Subsequent histological analysis of bladders from infected and noninfected mice showed that GML Gel was not toxic to bladder tissue.
Conclusion: Our results in this murine catheter infection model indicate that the newly formulated GML Gel may be useful in prevention and treatment of CAUTIs.
Hennepin Life Sciences:
P. Schlievert, Hennepin Life Sciences: Board Member , Consulting fee .