1412. Caspofungin and Anidulafungin Behave as Fungistatic Agents Against Candida auris.
Session: Poster Abstract Session: PK/PD Studies
Friday, October 5, 2018
Room: S Poster Hall
Posters
  • Caspofungin and anidulafungin behave as fungistatic agents against Candida auris.pdf (832.4 kB)
  • Background: Candida auris is an emerging multiresistant nosocomial pathogen responsible for
    outbreaks around the world. It is associated with therapeutic failure and high mortality
    rates. Echinocandins are the empiric treatment choice for C. auris infections. However,
    clinical reports show that some patients respond poorly to this therapy.
    The aim of this study was to determine the in vitro activity of Caspofungin and
    Anidulafungin against C. auris by time-kill curves method

    Methods: Twenty C. auris strains were studied. They were isolated from patients with proven
    invasive fungal infection. Susceptibility testing were performed following the Clinical
    and Laboratory Standards Institute (CLSI) M27-A3 and S4 documents. Time-killing
    experiments were conducted for 10 of the 20 isolates (in duplicate on 2 separate days)
    using RPMI-1640 buffered with MOPS. Caspofungin and anidulafungin tested
    concentration were 0.12, 0.25, 0.50, 1.00 and 8.00 µg/ml. The inoculum was adjusted
    to 1x105 CFU/ml using a Neubauer chamber. A 0.05 ml aliquot of each dilution was
    taken at different time points (0, 2, 4, 6, 8, 10, 24 and 48 h). These aliquots were
    serially diluted in sterile water, spread onto Sabouraud plates and incubated at 35°C to
    determine the numbers of CFU per milliliter. The killing kinetics and the fungicidal
    activity were analyzed by fitting the mean data at each time point to an exponential
    equation: Nt= N0 X e-kt (Nt viable yeasts at time t; N0 starting inoculum; K killing rate; t
    incubation time).

    Results: Anidulafungin and caspofungin MICs geometric means were 1.68 µg/ml (range: 0.5-8.0
    µg/ml) and 2.55 µg/ml (range 0.25-8.0 μg/mL), respectively. None of the drugs were
    able to reach fungicidal activity (no 99.9% inhibition). The mean time to reach 50%
    growth reduction were 1.74 ± 0.45 h and 5.30 ± 2.81 h for the MIC values of each
    strain for anidulafungin and caspofungin, respectively

    Conclusion: The tested echinocandins showed no in vitro fungicidal activity against C. auris at
    concentrations reached in serum despite strain´s MICs. Caspofungin exhibited a
    significant lowest killing rate.

    Catiana Dudiuk, PhD1,2, Indira Berrio, MD, MSc3,4, Laura Theill, PhD1,2, Soraya Morales-Lopez, PhD5,6, Soraya Salcedo, MD, MSc7, Jose Rodriguez, MD8,9, Soledad Gamarra, PhD1,2 and Guillermo Garcia-Effron, PhD1,2, (1)Laboratorio de Micología y Diagnóstico Molecular – Cátedra de Parasitología y Micología – Facultad de Bioquímica y Ciencias Biológicas – Universidad Nacional del Litoral, Santa Fe, Argentina, Santa Fe, Argentina, (2)Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), CCT-Santa Fe, Argentina, Santa Fe, Argentina, (3)Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas (CIB), Medellin, Colombia, (4)Hospital general de Medellin “Luz Castro de Gutiérrez” ESE, Medellín, Colombia, (5)Universidad Popular del Cesar, Valledupar, Colombia, Valledupar, Colombia, (6)Laboratorios Nancy Flórez García S.A.S, Valledupar, Colombia, Valledupar, Colombia, (7)Clínica General del Norte, Barranquilla, Colombia, (8)Hospital Rosario Pumarejo Lopez, Valledupar, Colombia, (9)Centro de Investigaciones Microbiológicas del Cesar – CIMCE Ltda, Valledupar, Colombia, Valledupar, Colombia

    Disclosures:

    C. Dudiuk, None

    I. Berrio, None

    L. Theill, None

    S. Morales-Lopez, None

    S. Salcedo, None

    J. Rodriguez, None

    S. Gamarra, None

    G. Garcia-Effron, None

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